Characterization of TsaR, an Oxygen-Sensitive LysR-Type Regulator for the Degradation of p -Toluenesulfonate in Comamonas testosteroni T-2
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چکیده
منابع مشابه
Characterization of TsaR, an oxygen-sensitive LysR-type regulator for the degradation of p-toluenesulfonate in Comamonas testosteroni T-2.
TsaR is the putative LysR-type regulator of the tsa operon (tsaMBCD) which encodes the first steps in the degradation of p-toluenesulfonate (TSA) in Comamonas testosteroni T-2. Transposon mutagenesis was used to knock out tsaR. The resulting mutant lacked the ability to grow with TSA and p-toluenecarboxylate (TCA). Reintroduction of tsaR in trans on an expression vector reconstituted growth wit...
متن کاملCharacterization of the p-toluenesulfonate operon tsaMBCD and tsaR in Comamonas testosteroni T-2.
Comamonas testosteroni T-2 uses a standard, if seldom examined, attack on an aromatic compound and oxygenates the side chain of p-toluenesulfonate (TS) (or p-toluenecarboxylate) to p-sulfobenzoate (or terephthalate) prior to complete oxidation. The expression of the first three catabolic enzymes in the pathway, the TS methyl-monooxygenase system (comprising reductase B and oxygenase M; TsaMB), ...
متن کاملMap of the IncP1beta plasmid pTSA encoding the widespread genes (tsa) for p-toluenesulfonate degradation in Comamonas testosteroni T-2.
The catabolic IncP1beta plasmid pTSA from Comamonas testosteroni T-2 was mapped by subtractive analysis of restriction digests, by sequencing outwards from the tsa operon (toluenesulfonate degradation), and by generating overlapping, long-distance-PCR amplification products. The plasmid was estimated to comprise 72 +/- 4 kb. The tsa region was found to be a composite transposon flanked by two I...
متن کاملIdentification and Characterization of the LysR - type Transcriptional 1 Regulator HsdR for Steroid - inducible Expression of the 3 α - Hydroxysteroid 2 Dehydrogenase / Carbonyl Reductase Gene in Comamonas testosteroni
Wenjie Gong, Guangming Xiong, Edmund Maser* 5 6 Institute of Toxicology and Pharmacology for Natural Scientists, University Medical School, Schleswig7 Holstein, Campus Kiel, Brunswiker Strasse 10,D-24105 Kiel, Germany 8 9 *Corresponding author: Prof. Edmund Maser, Tel.: +49-431-597-3540; fax: +49-431-597-3558; 10 E-mail address: [email protected] (E. Maser) 11 12 Running Title: HsdR positi...
متن کاملSteroid degradation in Comamonas testosteroni.
Steroid degradation by Comamonas testosteroni and Nocardia restrictus have been intensively studied for the purpose of obtaining materials for steroid drug synthesis. C. testosteroni degrades side chains and converts single/double bonds of certain steroid compounds to produce androsta-1,4-diene 3,17-dione or the derivative. Following 9α-hydroxylation leads to aromatization of the A-ring accompa...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 2003
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.69.4.2298-2305.2003